TESA (Testicular Sperm Aspiration)
Minimally Invasive Testicular Sperm Retrieval by Needle Aspiration
What is TESA (Testicular Sperm Aspiration)?
TESA (Testicular Sperm Aspiration) is a minimally invasive procedure for sperm retrieval in men with obstructive azoospermia or selected cases of non-obstructive azoospermia. A fine needle is passed through the scrotal skin into the testis, and testicular tissue or fluid is aspirated. The aspirate is examined immediately for the presence of spermatozoa. Retrieved sperm are used for ICSI (intracytoplasmic sperm injection) with the partner's oocytes. The procedure is performed under general or spinal anaesthesia with a hospital stay of Day Care. Success rates reach 50–70% in appropriately selected patients at experienced centres.
How the Procedure Works
Local Anaesthesia
Scrotum prepared; local anaesthesia injected into the spermatic cord and scrotal skin.
Needle Insertion
Fine gauge needle attached to a syringe passed through the scrotal skin into the testis.
Aspiration
Gentle suction applied while the needle is moved through the testicular parenchyma.
Embryologist Assessment
Aspirate examined under microscope by embryologist for presence of spermatozoa.
Multiple Sites if Needed
Procedure repeated in multiple sites or contralateral testis if initial aspirate is negative.
Outcomes
Who Needs This Treatment?
- →Least invasive sperm retrieval technique — needle only, no incision required.
- →Performed under local anaesthesia as a quick outpatient procedure.
- →Suitable for obstructive azoospermia where sperm production is normal.
- →Minimal post-procedural discomfort — most men return to work within 1–2 days.
- →Can be performed on the day of partner's egg collection for fresh ICSI.
- →Repeated procedures possible if needed; sperm can be cryopreserved.
"TESA is the simplest path to fatherhood for men with obstructive azoospermia. In 30 minutes under local anaesthesia, we retrieve sperm that allows ICSI and gives our patients the family they have been waiting for."
— — Dr. Vipin Reddy, Consultant Urologist, Andrologist & Renal Transplant Surgeon
Common Questions
Frequently Asked
Not sure which treatment is right for you?
Book a consultation with Dr. Vipin Reddy and get a personalised treatment plan.